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In this section: Laboratory Methods (Food)

BAM Media M36: Cell Growth Medium

Media Index for BAM

January 2001

Mix the following sterile solutions aseptically:

Minimal essential medium with Hanks' salts (MEMH)	1 liter
L-15 medium (Leibovitz) (containing 100 units penicillin G, 100 5g streptomycin, and 50 5g gentamicin per ml)	1 liter

Mix on magnetic stirrer, filter through 0.20 µm membrane, and dispense into sterile 2 liter Erlenmeyer flask. Final pH, 7.5. Cap and store at 5°C.

Just before use add:

Fetal bovine serum	200 ml
NaHCO₃ (7.5%)	50 ml

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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