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In this section: Laboratory Methods (Food)

BAM Media M34: Casamino Acids-Yeast Extract-Salts (CA-YE) Broth

Media Index for BAM

January 2001

(Gorbach)

Casamino acids	20 g
Yeast extract	6 g
NaCl	2.5 g
K₂HPO₄	8.71 g
Trace salts solution (below), optional	1 ml
Distilled water	1 liter

Adjust pH so that value after autoclaving is 8.5 ± 0.2. Autoclave 15 min at 121°C.

Trace salts solution (optional)

MgSO₄	50 g
MnCl₂	5 g
FeCl₂	5 g
Distilled water	1 liter

Suspend ingredients. Add enough 0.1 N H₂SO₄ to dissolve. Sterilize by filtration through 0.45 µm membrane. Add 1 ml to 1 liter base. Dispense 10 ml portions into 50 ml flasks.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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