U.S. flag An official website of the United States government
  1. Home
  2. Food
  3. Science & Research (Food)
  4. Laboratory Methods (Food)
  5. BAM Media M22: Blood Agar Base #2 (Difco)
  1. Laboratory Methods (Food)

BAM Media M22: Blood Agar Base #2 (Difco)

Media Index for BAM

January 2001

Proteose peptone 15 g
Liver digest 2.5 g
Yeast extract 5 g
NaCl 5 g
Agar 12 g
Distilled Water 1 liter

Autoclave at 121°C for 15 min. For blood agar, reduce water to 950 ml. Add 50 ml defibrinated (whole or lysed) horse blood and FBP (4 ml to agar + blood) after autoclaving and cooling to 48°C. Final pH, 7.4 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Back to Top