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In this section: Laboratory Methods (Food)

BAM Media M20a: Blood Agar Base

Media Index for BAM

January 2001

Infusion from beef heart	500 g
Tryptose	10 g
NaCl	5 g
Agar	15 g
Sheep blood (sterile, defibrinated)	50 ml
Distilled water	1 liter

Suspend ingredients except blood to dissolve. Autoclave 15 min at 121°C. Cool to 45-50°C. Add 50 ml blood (room temperature), and mix. Dispense into sterile petri dishes. Final pH, 6.8 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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