An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

In this section: Laboratory Methods (Food)

BAM Media M2: Acetamide Medium

Media Index for BAM

January 2001

Stock basal medium

KH₂PO₄	0.5 M	14 ml
K₂HPO₄	0.5 M	6 ml
Agar		0.5 g
Distilled water		400 ml

Heat with agitation to dissolve agar. Add 1 ml PR-CV (500× concentrate).

Stock acetamide, 1%

Acetamide	1 g
Distilled water	100 ml

Store over CHCl₃ in screw-cap container. Stable indefinitely at room temperature.

PR-CV (500× concentrate)

Phenol red	2 g
Crystal violet	0.2 g
Distilled water	200 ml

Add 5 N NaOH until ingredients are dissolved.

Final medium

Add 0.8 ml basal medium to 13 × 100 mm tube. Add 0.2 ml acetamide solution. Steam (100°C) 10 min. Cool.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top