Stock basal medium
|KH2PO4||0.5 M||14 ml|
|K2HPO4||0.5 M||6 ml|
|Distilled water||400 ml|
Heat with agitation to dissolve agar. Add 1 ml PR-CV (500× concentrate).
Stock acetamide, 1%
|Distilled water||100 ml|
Store over CHCl3 in screw-cap container. Stable indefinitely at room temperature.
PR-CV (500× concentrate)
|Phenol red||2 g|
|Crystal violet||0.2 g|
|Distilled water||200 ml|
Add 5 N NaOH until ingredients are dissolved.
Add 0.8 ml basal medium to 13 × 100 mm tube. Add 0.2 ml acetamide solution. Steam (100°C) 10 min. Cool.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.