BAM Media M199: Middlebrook 7H10 Agar
October 2023
|
Middlebrook 7H10 Agar base * |
19.47 g |
|---|---|
|
Middlebrook OADC Enrichment ** |
100 ml |
|
Glycerol |
5 ml |
|
Distilled water |
900 ml |
|
Middlebrook 7H10 Agar base * |
19.47 g |
|---|---|
|
Ammonium sulfate |
0.50 g |
|
Monopotassium phosphate |
1.50 g |
|
Disodium phosphate |
1.50 g |
|
Sodium citrate |
0.40 g |
|
Magnesium sulfate |
0.025 g |
|
Calcium chloride |
0.0005 g |
|
Zinc sulfate |
0.001 g |
|
Copper sulfate |
0.001 g |
|
L-Glutamic acid |
0.50 g |
|
Ferric ammonium citrate |
0.04 g |
|
Pyridoxine hydrochloride |
0.001 g |
|
Biotin |
0.0005 g |
|
Malachite green |
0.00025 g |
|
Agar |
15.00 g |
|
Middlebrook OADC Enrichment ** |
100 ml |
|---|---|
|
Bovine Albumin Fraction V |
5.00 g |
|
Dextrose |
2.00 g |
|
Catalase |
0.004 g |
|
Oleic acid |
0.05 g |
|
Sodium chloride |
0.85 g |
|
Distilled water |
100 ml |
* Middlebrook 7H10 Agar base are available from Fisher Scientific (Catalog No. DF0627-17-4), MilliporeSigma (SKU M0303), and other vendors with equivalent formulations.
** Middlebrook OADC Enrichment are available from Fisher Scientific (Catalog No. R450605), MilliporeSigma (SKU M0678), and other vendors with equivalent formulations.
Suspend 19.47 g of Middlebrook 7H10 Agar base in 900 ml of Distilled water containing 5 ml of Glycerol. Heat with agitation and boil for 1 minute to completely dissolve. Autoclave at 121oC for 10 minutes. Cool to 50-55oC and aseptically add 100 ml of Middlebrook OADC Enrichment. Mix and dispense. Final pH 6.6 +/- 0.2 at 25oC. Store the plates at 2-8oC with expiration in 90 days.