Prepare Sorbitol-MacConkey Agar (M-139) as directed. Once SMAC has been autoclaved and tempered, then add the 2 filter-sterilized additives below to obtain the final concentrations shown.
|Potassium tellurite||2.5mg/L (250 µL of 1% solution)*|
|Cefixime||0.05 mg/L (1mL of 50 mg/L solution in 95% EtOH)|
*Note: For 1% Potassium tellurite, store at 4° C for 1 month.
For Cefixime, store at -20° C for 1 year.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Updated and revised: 29-DEC-2000; 20-AUG-2010.