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In this section: Laboratory Methods (Food)

BAM Media M194: Tellurite Cefixime – Sorbitol MacConkey Agar (TC-SMAC)

Media Index for BAM

August 2010

Prepare Sorbitol-MacConkey Agar (M-139) as directed. Once SMAC has been autoclaved and tempered, then add the 2 filter-sterilized additives below to obtain the final concentrations shown.

Potassium tellurite	2.5mg/L (250 µL of 1% solution)*
Cefixime	0.05 mg/L (1mL of 50 mg/L solution in 95% EtOH)

*Note: For 1% Potassium tellurite, store at 4° C for 1 month.

For Cefixime, store at -20° C for 1 year.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Updated and revised: 29-DEC-2000; 20-AUG-2010.

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