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In this section: Laboratory Methods (Food)

BAM Media M192a: Modified Buffered Peptone water with pyruvate (mBPWp) and Acriflavin-Cefsulodin-Vancomycin (ACV) Supplement

Media Index for BAM

July 2009

Modified Buffered Peptone water with pyruvate (mBPWp)

Peptone	10.0 g
NaCl	5.0 g
Na₂HPO₄	3.6 g
KH₂PO₄	1.5 g
Casamino acids	5.0 g
Yeast extract	6.0 g
Lactose	10.0 g
Sodium Pyruvate	1.0g
Distilled water	1000 ml (Use 500 ml for 2× strength)

pH to 7.2 ± 0.2

Note: commercial BPW (20 g per L) can be substituted for the first four ingredients.

Sterilize by autoclaving.

Acriflavin-Cefsulodin-Vancomycin (ACV) Supplement for mBPWp

Supplement	Conc. in mBPWp	Conc. In stock	Amt. of stock/ 225mL
Acriflavin	10mg/L	1.125 g/ 500 mL	1 mL
Cefsulodin	10mg/L	1.125 g/ 500 mL	1 mL
Vancomycin	8mg/L	0.90 g/ 500 mL	1 mL

Filter, sterilize. Add 1mL of each to 225 mL mBPWp for selective overnight enrichment.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Updated and revised: 29-DEC-2000.

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