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In this section: Laboratory Methods (Food)

BAM Media M191: Vibrio vulnificus sucrose Agar (VPSA)

Media Index for BAM

May 2004

Tryptose	5.0 g
Tryptone	5.0 g
Yeast extract	7.0 g
Sucrose	10.0 g
NaCl	30.0 g
Bile salt #3	1.5 g
Bromthymol blue	0.025 g
Agar	15.0 g
Distilled water	1.0 L

Boil to dissolve ingredients and cool to 50-55°C. Adjust pH to 6.8 with 1.0 N NaOH and dispense into sterile petri dishes.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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