BAM Media M189: Cellobiose-Colistin (CC) Agar
May 2004
Solution 1
Peptone | 10 g |
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Beef extract | 5 g |
NaCl | 20 g |
1000× Dye stock solution* | 1 ml |
Agar | 15 g |
Distilled water | 900 ml |
Adjust to pH 7.6. Boil to dissolve agar. Cool to 48-55°C.
*1000× Dye stock solution
Bromthymol blue | 4.0 g |
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Cresol red | 4.0 g |
Ethanol, 95% | 100 ml |
For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes. Dissolve dyes in ethanol for 4% (w/v) stock solution. Using 1 ml of this solution per liter of mCPC agar gives 40 mg bromthymol blue and 40 mg cresol red per liter.
Solution 2
Cellobiose | 10 g |
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Colistin | 400,000 units |
Distilled water | 100 ml |
Dissolve cellobiose in distilled water by heating gently. Cool. Add antibiotics and filter sterilize.
Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, dark green to green-brown.
NOTE: This medium, like TCBS, is very inhibitory and does not require autoclaving. Medium may be stored 2 weeks at refrigeration temperatures.
Storage: limit: 2 wks, Temperature: 4°C