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In this section: Laboratory Methods (Food)

BAM Media M186: Trypicase (Tryptic) Soy Broth with ferrous sulfate

Media Index for BAM

January 2001

Trypticase peptone	17 g
Phytone peptone	3 g
NaCl	5 g
K₂HPO₄	2.5 g
Glucose	2.5 g
Ferrous sulfate	35 mg
Distilled water	1.0 liter

Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.

Original Source: adapted from Chapter 5, Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Created 2000-MAR-01

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