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In this section: Laboratory Methods (Food)

BAM Media M185: Malt Agar (MA)

Media Index for BAM

January 2001

Malt extract, powdered	20.0 g
Agar	20.0 g
Distilled water	1.0 liter

Mix ingredients, steam to dissolve agar and sterilize for 15 min at 121°C. Temper medium to 45°C and pour plates under aseptic conditions.

To prepare slants dispense 5-6 ml of steamed medium (before autoclaving) into each of several 16 × 125 mm screw-cap tubes, loosely cap tubes and sterilize as above. After autoclaving lay tubes in a slanting position and let them cool.

This medium is recommended as a general maintenance medium.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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