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In this section: Laboratory Methods (Food)

BAM Media M179: Xylose Lysine Desoxycholate (XLD) Agar

Media Index for BAM

January 2001, updated August 2010

Storage: May be stored up to 30 days under refrigeration (4 ± 2°C).

Yeast extract	3 g	Ferric ammonium citrate	0.8 g
L-lysine	5 g	Sodium thiosulfate	6.8 g
Xylose	3.75 g	NaCl	5 g
Lactose	7.5 g	Agar	15 g
Sucrose	7.5 g	Phenol red	0.08 g
Sodium desoxycholate	2.5 g	Distilled water	1 liter

Heat with agitation just until medium boils. Do not overheat. Pour into plates when medium has cooled to 50°C. Let dry about 2 h with covers partially removed. Then close plates. Final pH, 7.4 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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