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  1. Laboratory Methods (Food)

BAM Media M179: Xylose Lysine Desoxycholate (XLD) Agar

January 2001, updated August 2010

Bacteriological Analytical Manual

Xylose Lysine Desoxycholate (XLD) Agar

StorageMay be stored up to 30 days under refrigeration (4 ± 2°C).
Yeast extract3 gFerric ammonium citrate0.8 g
L-lysine5 gSodium thiosulfate6.8 g
Xylose3.75 gNaCl5 g
Lactose7.5 gAgar15 g
Sucrose7.5 gPhenol red0.08 g
Sodium desoxycholate2.5 gDistilled water1 liter

Heat with agitation just until medium boils. Do not overheat. Pour into plates when medium has cooled to 50°C. Let dry about 2 h with covers partially removed. Then close plates. Final pH, 7.4 ± 0.2.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.