January 2001, updated August 2010
Xylose Lysine Desoxycholate (XLD) Agar
|Storage||May be stored up to 30 days under refrigeration (4 ± 2°C).|
|Yeast extract||3 g||Ferric ammonium citrate||0.8 g|
|L-lysine||5 g||Sodium thiosulfate||6.8 g|
|Xylose||3.75 g||NaCl||5 g|
|Lactose||7.5 g||Agar||15 g|
|Sucrose||7.5 g||Phenol red||0.08 g|
|Sodium desoxycholate||2.5 g||Distilled water||1 liter|
Heat with agitation just until medium boils. Do not overheat. Pour into plates when medium has cooled to 50°C. Let dry about 2 h with covers partially removed. Then close plates. Final pH, 7.4 ± 0.2.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.