BAM Media M176: Vogel-Johnson (VJ) Agar
January 2001
| Trypticase | 10 g |
| Yeast extract | 5 g |
| Mannitol | 10 g |
| K2HPO4 | 5 g |
| Lithium chloride | 5 g |
| Glycine | 10 g |
| Phenol red | 0.025 g |
| Agar | 15 g |
| Distilled water | 1 liter |
Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Cool to 50°C. Add 20 ml Chapman tellurite solution, 1%, commercially available from Difco Laboratories, Detroit, MI. Mix and pour into plates. Final pH, 7.2 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.