Violet Red Bile Agar (VRBA)
|Yeast extract||3.0 g|
|Peptone or gelysate||7.0 g|
|Bile salts or bile salts No. 3||1.5 g|
|Neutral red||0.03 g|
|Crystal violet||0.002 g|
|Distilled water||1.0 liter|
Suspend ingredients in distilled water and let stand for a few min. Mix thoroughly and adjust to pH 7.4 ± 0.2. Heat with agitation and boil for 2 min. Do not sterilize. Before use, cool to 45°C and use as a plating medium. After solidification, add a cover layer above the agar of approximately 3.0 to 4.0 ml to prevent surface growth and spreading of colonies.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.