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  1. Laboratory Methods (Food)

BAM Media M174: Violet Red Bile Agar (VRBA)

January 2001

Bacteriological Analytical Manual

Violet Red Bile Agar (VRBA)

Yeast extract3.0 g
Peptone or gelysate7.0 g
NaCl5.0 g
Bile salts or bile salts No. 31.5 g
Lactose10.0 g
Neutral red0.03 g
Crystal violet0.002 g
Agar15.0 g
Distilled water1.0 liter

Suspend ingredients in distilled water and let stand for a few min. Mix thoroughly and adjust to pH 7.4 ± 0.2. Heat with agitation and boil for 2 min. Do not sterilize. Before use, cool to 45°C and use as a plating medium. After solidification, add a cover layer above the agar of approximately 3.0 to 4.0 ml to prevent surface growth and spreading of colonies.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.