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In this section: Laboratory Methods (Food)

BAM Media M171: Urea Broth

Media Index for BAM

January 2001; Updated February 2008

Urea	20 g
Yeast extract	0.1 g
Na₂HPO₄	9.5 g
KH₂PO₄	9.1 g
Phenol red	0.01 g
Distilled water	1 liter

Dissolve ingredients in distilled water. DO NOT HEAT. Sterilize by filtration through 0.45 µm membrane. Aseptically dispense 1.5-3.0 ml portions to 13 × 100 mm sterile test tubes. Final pH, 6.8 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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