Tryptose-Sulfite-Cycloserine (TSC) Agar
|Yeast extract||5 g|
|Ferric ammonium citrate
(NF Brown Pearls)
|Sodium metabisulfite||1 g|
|Distilled water||900 ml|
Heat with agitation to dissolve. Adjust pH to 7.6 ± 0.2. Dispense 250 ml portions to 500 ml flasks. Autoclave 15 min at 121°C. Maintain medium at 50°C before use. Dehydrated SFP agar base (Difco) is satisfactory for base.
Dissolve 1 g D-cycloserine (white crystalline powder) in 200 ml of distilled water. Sterilize by filtration and store at 4°C until use. D-cycloserine powder is available from Sigma Chemical Co., St. Louis, MO.
For pour plates, add 20 ml of D-cycloserine solution to 250 ml base. To prepare prepoured plates containing egg yolk, also add 20 ml of 50% egg yolk emulsion (M51). Mix well and dispense 18 ml into 15 × 100 mm petri dishes. Cover plates with a towel and let dry overnight at room temperature before use.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.