|Beef extract||3 g|
|Distilled water||1 liter|
Suspend ingredients in distilled water, mix thoroughly, and heat with occasional agitation. Boil about 1 min. Fill 16 × 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave 15 min at 121°C. Before media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.