|Yeast extract||1 g|
|Bromcresol purple||0.04 g|
|Distilled water||1 liter|
Dissolve agar with heat and gentle agitation. Adjust pH to 7.0 ± 0.2. Fill 16 × 125 mm tubes 2/3 full. Autoclave 20 min at 115°C. Before use, steam medium 10-15 min. Solidify by placing tubes in ice water.
*Glucose and mannitol are the carbohydrates used for identification of Staphylococcus aureus.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.