BAM Media M165: Tryptone Yeast Extract Agar
January 2001
| Tryptone | 10 g |
| Yeast extract | 1 g |
| *Carbohydrate | 10 g |
| Bromcresol purple | 0.04 g |
| Agar | 2 g |
| Distilled water | 1 liter |
Dissolve agar with heat and gentle agitation. Adjust pH to 7.0 ± 0.2. Fill 16 × 125 mm tubes 2/3 full. Autoclave 20 min at 115°C. Before use, steam medium 10-15 min. Solidify by placing tubes in ice water.
*Glucose and mannitol are the carbohydrates used for identification of Staphylococcus aureus.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.