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In this section: Laboratory Methods (Food)

BAM Media M16: Arginine-Glucose Slant (AGS)

Media Index for BAM

January 2001

Peptone	5 g
Yeast extract	3 g
Tryptone	10 g
NaCl	20 g
Glucose	1 g
L-Arginine (hydrochloride)	5 g
Ferric ammonium citrate	0.5 g
Sodium thiosulfate	0.3 g
Bromocresol purple	0.02 g
Agar	13.5 g
Distilled water	1 liter

Suspend ingredients in distilled water and boil to dissolve. Dispense into tubes (for 13 × 100 mm tubes use 5 ml). Autoclave 10-12 min at 121°C. After sterilization, solidify as slants. Final pH, 6.8-7.0.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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