|Trypticase soy broth||30.0 g|
|Bile salts No. 3||1.5 g|
|Dipotassium phosphate||1.5 g|
|Novobiocin solution (R50)||0.2 ml|
|Deionized water||1 liter|
Autoclave mTSB without novobiocin and let cool to room temperature. Final pH, 7.4 +/- 0.2. For chapter 24, gene probe method, add novobiocin just before adding food.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Revisions: January 28, 2002 Required pH specified.