An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

In this section: Laboratory Methods (Food)

BAM Media M156: Trypticase Soy Broth Modified (mTSB)

Media Index for BAM

January 2002

Trypticase soy broth	30.0 g
Bile salts No. 3	1.5 g
Dipotassium phosphate	1.5 g
Novobiocin solution (R50)	0.2 ml
Deionized water	1 liter

Autoclave mTSB without novobiocin and let cool to room temperature. Final pH, 7.4 +/- 0.2. For chapter 24, gene probe method, add novobiocin just before adding food.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
Revisions: January 28, 2002 Required pH specified.

Top