BAM Media M154a: Trypticase (Tryptic) Soy Broth with 10% NaCl and 1% Sodium Pyruvate
January 2001
| Trypticase or tryptose (pancreatic digest of casein) |
17 g |
| Phytone (papaic digest of soya meal) |
3 g |
| NaCl | 100 g |
| K2HPO4 | 2.5 g |
| Dextrose | 2.5 g |
| Sodium pyruvate | 10 g |
| Distilled water | 1 liter |
Dehydrated trypticase or tryptic soy broth is satisfactory with 95 g NaCl and 10 g sodium pyruvate added per liter. Adjust to pH 7.3. Heat gently if necessary. Dispense 10 ml into 16 × 150 mm tubes. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2. Store up to 1 month at 4 ± 1°C.
| Storage | |
|---|---|
| Temperature | 4 ± 1° C |
| Duration | 1 month |
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.