|Trypticase peptone||17 g|
|Phytone peptone||3 g|
|Distilled water||1 liter|
Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.
For trypticase soy broth without glucose, prepare as above, but omit 2.5 g glucose.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.