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  1. Laboratory Methods (Food)

BAM Media M152: Trypticase (Tryptic) Soy Agar

January 2001

Bacteriological Analytical Manual

Trypticase peptone 15 g
Phytone peptone 5 g
NaCl 5 g
Agar 15 g
Distilled water 1 liter

Heat with agitation to dissolve agar. Boil 1 min. Dispense into suitable tubes or flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.