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BAM Media M151: Trypticase-Peptone-Glucose-Yeast Extract Broth (TPGY)

January 2001

Bacteriological Analytical Manual

Trypticase 50 g
Peptone 5 g
Yeast extract 20 g
Dextrose 4 g
Sodium thioglycollate 1 g
Distilled water l liter

Dissolve solid ingredients and dispense 15 ml in 20 × 150 mm tubes. Autoclave tubes 10 min at 121°C. Final pH, 7.0 ± 0.2. Refrigerate at 5°C.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.