BAM Media M151: Trypticase-Peptone-Glucose-Yeast Extract Broth (TPGY)
January 2001
Trypticase | 50 g |
Peptone | 5 g |
Yeast extract | 20 g |
Dextrose | 4 g |
Sodium thioglycollate | 1 g |
Distilled water | l liter |
Dissolve solid ingredients and dispense 15 ml in 20 × 150 mm tubes. Autoclave tubes 10 min at 121°C. Final pH, 7.0 ± 0.2. Refrigerate at 5°C.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.