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In this section: Laboratory Methods (Food)

BAM Media M150: Trypticase Novobiocin (TN) Broth

Media Index for BAM

January 2001

Trypticase soy broth	30 g
Bile salts No. 3	1.5 g
Dipotassium phosphate	1.5 g
Novobiocin	20 mg
Distilled water	1 liter

Dissolve all ingredients except novobiocin by heating and stirring; autoclave at 121°C for 15 min. Prepare stock solution of novobiocin by adding 20 mg monosodium novobiocin per ml of distilled water. Filter-sterilize.

Make fresh stock each time of use, or store frozen at -10°C in the dark (compound is light-sensitive) for not more than 1 month (half-life is several months at 4°C). Add 1 ml stock solution per liter of medium.

Make up fresh or
Storage:	Frozen [-10°C] dark conditions up to 1 month

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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