|Trypticase soy broth||30 g|
|Bile salts No. 3||1.5 g|
|Dipotassium phosphate||1.5 g|
|Distilled water||1 liter|
Dissolve all ingredients except novobiocin by heating and stirring; autoclave at 121°C for 15 min. Prepare stock solution of novobiocin by adding 20 mg monosodium novobiocin per ml of distilled water. Filter-sterilize.
Make fresh stock each time of use, or store frozen at -10°C in the dark (compound is light-sensitive) for not more than 1 month (half-life is several months at 4°C). Add 1 ml stock solution per liter of medium.
|Make up fresh
up to 1 month
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.