BAM Media M148: Toluidine Blue-DNA Agar
January 2001
Deoxyribonucleic acid (DNA) | 0.3 g |
---|---|
Agar | 10 g |
CaCl2 (anhydrous) | 1.1 mg |
NaCl | 10 g |
Toluidine blue O | 0.083 g |
Tris (hydroxymethyl) aminomethane | 6.1 g |
Distilled water | 1 liter |
Dissolve Tris (hydroxymethyl) aminomethane in 1 liter distilled water. Adjust pH to 9.0. Add the remaining ingredients except toluidine blue O and heat to boiling to dissolve. Dissolve toluidine blue O in medium. Dispense to rubber-stoppered flasks. Sterilization is not necessary if used immediately. The sterile medium is stable at room temperature for 4 months and is satisfactory after several melting cycles.
Storage | room temperature |
Shelf life | 4 months |
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.