|Deoxyribonucleic acid (DNA)||0.3 g|
|CaCl2 (anhydrous)||1.1 mg|
|Toluidine blue O||0.083 g|
|Tris (hydroxymethyl) aminomethane||6.1 g|
|Distilled water||1 liter|
Dissolve Tris (hydroxymethyl) aminomethane in 1 liter distilled water. Adjust pH to 9.0. Add the remaining ingredients except toluidine blue O and heat to boiling to dissolve. Dissolve toluidine blue O in medium. Dispense to rubber-stoppered flasks. Sterilization is not necessary if used immediately. The sterile medium is stable at room temperature for 4 months and is satisfactory after several melting cycles.
|Shelf life||4 months|
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.