Bacteriological Analytical Manual
|Agar (granulated)||0.75 g|
|Yeast extract||5 g|
|Sodium thioglycollate or
sodium solution (1:1000), fresh
|Distilled water||1 liter|
Mix L-cystine, NaCl, dextrose, yeast extract, and tryptone with 1 liter water. Heat in Arnold steamer or water bath until ingredients are dissolved. Dissolve sodium thioglycollate or thioglycollic acid in solution and adjust pH so that value after sterilization is 7.1 ± 0.2. Add sodium resazurin solution, mix, and autoclave 20 min at 121°C. If commercial media are used, dispense 10 ml portions to 16 × 150 mm tubes and autoclave 15 min at 121°C.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.