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  1. Laboratory Methods (Food)

BAM Media M140: Sporulation Broth (for Clostridium perfringens)

January 2001

Bacteriological Analytical Manual

Polypeptone 15 g
Yeast extract 3 g
Starch, soluble 3 g
MgSO4 (anhydrous) 0.1 g
Sodium thioglycollate 1 g
Na2HPO4 11 g
Distilled water 1 liter

Adjust pH to 7.8 ± 0.1. Dispense 15 ml portions into 20 × 150 mm screw-cap tubes. Autoclave 15 min at 121°C.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.