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In this section: Laboratory Methods (Food)

BAM Media M139: Sorbitol-MacConkey Agar

Media Index for BAM

January 2001

Peptone or gelysate	17.0 g
Protease peptone No. 3 or polypeptone	3.0 g
Sorbitol	10.0 g
Bile salts, purified	1.5 g
NaCl	5.0 g
Agar	13.5 g
Neutral red	0.03 g
Crystal violet	0.001 g
Distilled water	1 liter

Dissolve ingredients in distilled water by heating with stirring. Autoclave 15 min at 121°C. Final pH, 7.1 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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