|Sodium citrate*||2 g|
|Bromthymol blue||0.08 g|
|Distilled water||1 liter|
Heat gently with occasional agitation. Boil 1-2 min until agar dissolves. Fill 13 × 100 or 16 × 150 mm screw-cap tubes 1/3 full. Autoclave 15 min at 121°C. Before medium solidifies, incline tubes to obtain 4-5 cm slants and 2-3 cm butts. Final pH, 6.8 ± 0.2.
*Difco does not specify waters of hydration.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.