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In this section: Laboratory Methods (Food)

BAM Media M138: Simmons Citrate Agar

Media Index for BAM

January 2001

Sodium citrate*	2 g
NaCl	5 g
K₂HPO₄	1 g
NH₄H₂PO₄	1 g
MgSO₄	0.2 g
Bromthymol blue	0.08 g
Agar	15 g
Distilled water	1 liter

Heat gently with occasional agitation. Boil 1-2 min until agar dissolves. Fill 13 × 100 or 16 × 150 mm screw-cap tubes 1/3 full. Autoclave 15 min at 121°C. Before medium solidifies, incline tubes to obtain 4-5 cm slants and 2-3 cm butts. Final pH, 6.8 ± 0.2.

*Difco does not specify waters of hydration.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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