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In this section: Laboratory Methods (Food)

BAM Media M136: Shigella Broth

Media Index for BAM

January 2001

Base

Tryptone	20 g
K₂HPO₄	2 g
KH₂PO₄	2 g
NaCl	5 g
Glucose	1 g
Tween 80	1.5 ml
Distilled water	1 liter

Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.

Novobiocin solution

Weigh 50 mg novobiocin into 1 liter distilled water.

Sterilize by filtration through 0.45 µm membrane. Add 2.5 ml concentrate to 225 ml base.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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