An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

In this section: Laboratory Methods (Food)

BAM Media M136: Shigella Broth

January 2001

Base

Tryptone	20 g
K₂HPO₄	2 g
KH₂PO₄	2 g
NaCl	5 g
Glucose	1 g
Tween 80	1.5 ml
Distilled water	1 liter

Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.

Novobiocin solution

Weigh 50 mg novobiocin into 1 liter distilled water.

Sterilize by filtration through 0.45 µm membrane. Add 2.5 ml concentrate to 225 ml base.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top