U.S. flag An official website of the United States government

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

U.S. Food and Drug Administration
  1. Home
  2. Food
  3. Science & Research (Food)
  4. Laboratory Methods (Food)
  5. BAM Media M134: Selenite Cystine Broth
  1. Laboratory Methods (Food)

BAM Media M134: Selenite Cystine Broth

Media Index for BAM

January 2001

Medium 1 (modification of Leifson's formulation for selenite broth)

Tryptone or polypeptone 5 g
Lactose 4 g
Sodium acid selenite (NaHSeO3) 4 g
Na2HPO4 10 g
L-Cystine 0.01 g
Distilled water 1 liter

Heat to boiling to dissolve. Dispense 10 ml portions into sterile 16 × 150 mm test tubes. Heat 10 min in flowing steam. DO NOT AUTOCLAVE. Final pH, 7.0 ± 0.2. The medium is not sterile. Use same day as prepared.

Medium 2 (North-Bartram modification)

Polypeptone 5 g
Lactose 4 g
Sodium acid selenite (NaHSeO3) 4 g
Na2HPO4 5.5 g
KH2PO4 4.5 g
L-Cystine 0.01 g
Distilled water 1 liter

Heat with agitation to dissolve. Dispense 10 ml portions into sterile 16 × 150 mm test tubes. Heat 10 min in flowing steam. DO NOT AUTOCLAVE. Use same day as prepared.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Back to Top