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BAM Media M134: Selenite Cystine Broth

BAM Media M134: Selenite Cystine Broth

January 2001

Bacteriological Analytical Manual

Medium 1 (modification of Leifson's formulation for selenite broth)

Tryptone or polypeptone 5 g
Lactose 4 g
Sodium acid selenite (NaHSeO3) 4 g
Na2HPO4 10 g
L-Cystine 0.01 g
Distilled water 1 liter

Heat to boiling to dissolve. Dispense 10 ml portions into sterile 16 × 150 mm test tubes. Heat 10 min in flowing steam. DO NOT AUTOCLAVE. Final pH, 7.0 ± 0.2. The medium is not sterile. Use same day as prepared.

Medium 2 (North-Bartram modification)

Polypeptone 5 g
Lactose 4 g
Sodium acid selenite (NaHSeO3) 4 g
Na2HPO4 5.5 g
KH2PO4 4.5 g
L-Cystine 0.01 g
Distilled water 1 liter

Heat with agitation to dissolve. Dispense 10 ml portions into sterile 16 × 150 mm test tubes. Heat 10 min in flowing steam. DO NOT AUTOCLAVE. Use same day as prepared.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.