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  1. Laboratory Methods (Food)

BAM Media M132: Rappaport-Vassiliadis Medium

Media Index for BAM

January 2001

Broth base

Tryptone 5 g
NaCl 8 g
KH2PO4 1.6 g
Distilled water 1 liter

Magnesium chloride solution

MgCl2·6H2O 400 g
Distilled water 1 liter

Malachite green oxalate solution

Malachite green oxalate 0.4 g
Distilled water 100 ml

To prepare the complete medium, combine 1000 ml broth base, 100 ml magnesium chloride solution, and 10 ml malachite green oxalate solution (total volume of complete medium is 1110 ml). Broth base must be prepared on same day that components are combined to make complete medium. Magnesium chloride solution may be stored in dark bottle at room temperature up to 1 year. To prepare solution, dissolve entire contents of MgCl2·6H2O from newly opened container according to formula, because this salt is very hygroscopic. Malachite green oxalate solution may be stored in dark bottle at room temperature up to 6 months. Merck analytically pure malachite green oxalate is recommended because other brands may not be equally effective. Dispense 10 ml volumes of complete medium into 16 × 150 mm test tubes. Autoclave 15 min at 115°C. Final pH, 5.5 ± 0.2. Store in refrigerator and use within 1 month.

This medium must be made from its individual ingredients. Use of commercially available dehydrated media is not recommended. Users of this medium should be aware that there are formulations and incubation temperatures for this medium other than those recommended in this manual.

Storage Conditions: Refrigerator [4-8°C]
Shelf Life: 1 month

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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