Use Difco product, if available.
|Proteose peptone No. 3 (Difco)||20.0 g|
|Tryptone (Difco)||10.0 g|
|Dipotassium phosphate||1.5 g|
|Magnesium sulfate||0.73 g|
|Glycerol (Difco)||10.0 g|
|Distilled water||1 liter|
Add medium powder and glycerol to water; mix. Heat to boiling to dissolve ingredients. Autoclave at 121°C for 15 min. Final pH, 7.0.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.