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  5. BAM Media M126: Potassium Cyanide (KCN) Broth
  1. Laboratory Methods (Food)

BAM Media M126: Potassium Cyanide (KCN) Broth

Media Index for BAM

January 2001

Bacteriological Analytical Manual

*Potassium cyanide (KCN) 0.5 g
Proteose peptone No. 3 or polypeptone 3 g
NaCl  5 g
KH2PO4 0.225 g
Na2HPO4 5.64 g
Distilled water 1 liter

Dissolve above ingredients except potassium cyanide and autoclave 15 min at 121°C. Cool and refrigerate at 5-8°C. Final pH, 7.6 ± 0.2.

Prepare KCN stock solution by dissolving 0.5 g KCN in 100 ml sterile distilled water cooled to 5-8°C.

Using bulb pipetter, add 15 ml cold KCN stock solution to 1 liter cold, sterile base.

DO NOT PIPET BY MOUTH!!!
Handle with gloves!!

Mix and aseptically dispense 1.0-1.5 ml portions to 13 × 100 mm sterile tubes. Using aseptic technique, stopper tubes with No. 2 corks impregnated with paraffin. Prepare corks by boiling in paraffin about 5 min. Place corks in tubes so that paraffin does not flow into broth but forms a seal between rim of tubes and cork. Store tubes at 5-8°C no longer than 2 weeks before use.

Caution:

*POISON!!
DO NOT PIPET BY MOUTH!
Use protective equipment/gloves when handling!
STORAGE CONDITIONS 5-8°
Retain no longer than two weeks before use.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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