BAM Media M118a: PALCAM Listeria Selective Agar
January 2001
Basal medium
| Peptone | 23 g |
|---|---|
| Starch | 1 g |
| NaCl | 5 g |
| Columbia agar | 13 g |
| Mannitol | 10 g |
| Ferric ammonium citrate | 0.5 g |
| Esculin (aesculin) | 0.8 g |
| Dextrose (glucose) | 0.5 g |
| Lithium chloride | 15.0 g |
| Phenol red | 0.08 g |
| Distilled water | 1000 ml |
Selective agents
| Polymyxin B sulfate | 10 mg |
|---|---|
| Acriflavin | 5 mg |
| Ceftazidine | 20 mg |
| Distilled water | 2 ml |
To make 500 ml of medium, weigh 34.4 g basal medium powder (all ingredients except the three selective agents) and suspend in 500 ml distilled water. Sterilize by autoclaving at 121°C for 15 min. Dissolve the selective agent supplement mixture in sterile distilled water at 17.5 mg/ml and filter sterilize. Add 1 ml selective agent supplement solution to 500 ml sterile basal medium that has been cooled to 50°C. Mix gently and pour plates. Final pH, 7.2 ± 0.1.
*NOTE: The basal medium powder and lyophilized supplement mixture are available commercially; follow the manufacturer's instructions for preparing the medium.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.