BAM Media M118: Oxford Medium
January 2001
| Columbia blood agar base | 39.0 g |
| Esculin | 1.0 g |
| Ferric ammonium citrate | 0.5 g |
| Lithium chloride | 15.0 g |
| Cycloheximide | 0.4 g |
| Colistin sulfate | 0.02 g |
| Acriflavin | 0.005 g |
| Cefotetan | 0.002 g |
| Fosfomycin | 0.010 g |
| Distilled water | 1 liter |
Add the 55.5 g of the first 4 components (basal medium) to 1 liter distilled water. Bring gently to boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 min. Cool to 50°C and aseptically add supplement, mix, and pour into sterile petri dishes. To prepare supplement, dissolve cycloheximide, colistin sulfate, acriflavin, cefotetan, and fosfomycin in 10 ml of 1:1 mixture of ethanol and distilled water. Filter-sterilize supplement before use. Oxford basal medium and supplement mixture are available commercially.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.