|Beef extract||3 g|
|Distilled water||1 liter|
Heat to boiling to dissolve ingredients. Dispense into tubes or flasks. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2. If used as base for blood agar, add 8 g NaCl to prevent hemolysis of blood cells.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.