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  1. Laboratory Methods (Food)

BAM Media M107: Mueller-Hinton Agar

January 2001

Beef, infusion from 300 g
Acidicase Peptone (BBL)
or
Casamino Acids (Difco)
17.5 g
Starch 1.5 g
Agar 17 g
Distilled water 1 liter

Heat to boiling for 1 min. Autoclave 15 min at 116°C. Final pH, 7.3 ± 0.2.

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.