|Beef extract||3 g|
|Peptone (Difco)||5 g|
|Glycerin (reagent grade)||5 ml|
|Distilled water||1 liter|
Dissolve all ingredients except agar. Adjust pH to 7.3 ± 0.1. Add agar and heat to dissolve. Dispense 11 ml portions into 16 > 150 mm tubes. Autoclave 15 min at 121°C. If not used within 4 h, heat 10 min in boiling water or flowing steam. Chill in cold water.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.