BAM Media M102: Motility-Nitrate Medium, Buffered (for C. perfringens)
January 2001
Beef extract | 3 g |
Peptone (Difco) | 5 g |
KNO3 | 1 g |
Na2HPO3 | 2.5 g |
Agar | 3 g |
Galactose | 5 g |
Glycerin (reagent grade) | 5 ml |
Distilled water | 1 liter |
Dissolve all ingredients except agar. Adjust pH to 7.3 ± 0.1. Add agar and heat to dissolve. Dispense 11 ml portions into 16 > 150 mm tubes. Autoclave 15 min at 121°C. If not used within 4 h, heat 10 min in boiling water or flowing steam. Chill in cold water.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.