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In this section: Laboratory Methods (Food)

BAM Media M102: Motility-Nitrate Medium, Buffered (for C. perfringens)

Media Index for BAM

January 2001

Beef extract	3 g
Peptone (Difco)	5 g
KNO₃	1 g
Na₂HPO₃	2.5 g
Agar	3 g
Galactose	5 g
Glycerin (reagent grade)	5 ml
Distilled water	1 liter

Dissolve all ingredients except agar. Adjust pH to 7.3 ± 0.1. Add agar and heat to dissolve. Dispense 11 ml portions into 16 > 150 mm tubes. Autoclave 15 min at 121°C. If not used within 4 h, heat 10 min in boiling water or flowing steam. Chill in cold water.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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