|Heart infusion agar (Difco)||8 g|
|Potassium (or sodium) nitrate||1 g|
|Distilled water||1 liter|
Heat with agitation to dissolve agar. Dispense 4 ml portions into 13 × 100 mm screw-cap tubes. Autoclave 15 min at 121°C. Available as prepared medium from BBL and Scott.
NOTE: Make sure nitrates are nitrite-free.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.