|*Triton X-100 (Rohm & Haas)||1 ml|
|Distilled water||1 liter|
Dissolve ingredients in 1 liter distilled water. Adjust pH to 6.9 ± 0.1. Dispense 10 ml portions of single strength broth into 18 × 150 mm tubes containing inverted fermentation vials. For double strength broth, use 22 × 175 mm tubes containing inverted fermentation vials. Medium may be cloudy before sterilization. Autoclave 10 min at 121°C.
Store in dark up to 7 days.
(Commercially available A-1 medium is unacceptable)
|Storage||dark, up to 7 days|
*Triton X-100 may be purchased from Fisher Scientific Company, Fairlawn, NJ 07410.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.