Currently Funded Opportunities
Infrastructure funding is key to making sure that the network can function and that laboratories can work to support Vet-LIRN’s mission. These awards facilitate participation in Vet-LIRN activities such as consumer complaint response, emergency exercises, proficiency tests, and laboratory accreditation. The agreements also increase the agency’s capability to analyze an increased number of samples in the event of animal food- or drug-related illnesses or other large-scale emergency events that require increased testing of implicated diagnostic or animal food samples. Cooperative agreements allow network laboratories to request additional funds if they are participating in a specific Vet-LIRN project, such as the Antimicrobial Resistance (AMR) monitoring program or if they are conducting whole-genome sequencing (WGS) work, or if their caseload is particularly heavy.
The cooperative agreement is intended to support projects or equipment grants to help investigate potential issues with animal foods, as well as support work related to antimicrobial resistance and antimicrobial stewardship. Vet-LIRN Laboratories may apply for funding for research related to emerging public food safety issues identified by the Vet-LIRN and for equipment and personnel necessary to expand laboratory capability and capacity. This work may include the development and validation of new methods or work to support efforts related to antimicrobial resistance or antimicrobial stewardship. In 2021, a Notice of Special Interest was added to this announcement to request applications related to COVID-19 animal diagnostic work.
Figure 1. What are the steps during a Blinded Method Test?
Blinded Method Test
Testing animal diagnostic specimens enables Vet-LIRN to complete consumer complaints about animal food or drugs. The Capacity Building grants help Vet-LIRN ensure that the data from our laboratories is derived from methods that have been evaluated. Although Vet-LIRN methods are not regulatory methods, the FDA Method Validation Guidelines are generally followed during the method development and evaluation. Most of the projects have the following phases:
- Method-Originating Laboratory (MO) – Cooperative-Grant Recipient
- Method development/optimization.
- Validation by the single MO laboratory.
- Vet-LIRN evaluates the method performance in the MO laboratory through exercises called Blinded Method Tests (BMTs). Vet-LIRN prepares samples, and the MO laboratory analyzes blinded samples.
- MO Laboratory
- Method transfers to other Vet-LIRN collaborating laboratories.
- Vet-LIRN evaluates the method performance in multiple laboratories with a Blinded Method Test.
The methods developed by our Vet-LIRN laboratories are extensively evaluated and will be
- published in a scientific journal, and
- adopted and used by Vet-LIRN laboratories for animal diagnostic purposes.
Highlighted methods that completed BMTs 2020-2022 include
- Texas A&M – Evaluation of PCR method to detect Campylobacter in bovine feces. The method developed to detect Campylobacter in canine feces was expanded for use in bovine feces. The method replaces aging real-time PCR equipment with new equipment compatible with that used in other veterinary diagnostic laboratories for detection. Evaluation of the method for the expanded matrix will allow rapid, efficient detection of pathogens of interest to support outbreak response.
- University of Pennsylvania - Quantitation of aflatoxin B1 in fish food using lateral flow assay, which is based on the CHARM system. Mold can produce aflatoxin B1 in food ingredients such as grains (e.g. corn, wheat, barley), rice, nuts, and fruits. Aflatoxin B1 is the most toxic aflatoxin and is known for its mutagenic, carcinogenic, teratogenic, and immunosuppressive impact. It can cause death or intoxication in fish resulting in reduced growth rate and immunosuppression. Therefore, fast methods to identify this toxicant in fish food are desirable. The method was evaluated in both single-laboratory and multi-laboratory BMTs. In both BMTs samples were prepared by Vet-LIRN and analyzed in four collaborating laboratories.
- Kansas State University - Quantitation of the irradiation marker 2-DCB in dried chicken using GC-MS. Some manufacturers irradiate pet foods to kill bacterial and viral pathogens and extend food shelf life. However, improper food irradiation can result in microbial contamination of food products. Thus, having an effective method for determining irradiation dose is important to determine whether foods are properly irradiated.
- University of Pennsylvania Detection of Clostridioides difficile in dog feces. Collaborator: PA laboratory
- Purdue University - Determination of three organophosphate pesticides (terbufos, diazinon and parathion) in bovine blood using GC-MS. Collaborator: IN laboratory