Seongwon
Nho
Ph.D.
Leadership Role
Staff Fellow — Division of Microbiology
Seongwon Nho, Ph.D.
(870) 543-7121
NCTRResearch@fda.hhs.gov
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About | Publications | Lab Members
Background
Dr. Seongwon Nho graduated with a Doctor of Veterinary Medicine degree from Gyeongsang National University (GNU), South Korea. He then completed a Ph.D. in veterinary medicine at GNU, where his research focused on the proteomic response to infection in teleosts and genome analysis of pathogens. Following his doctoral studies, he pursued postdoctoral training at Mississippi State University, specializing in aquatic animal health and food safety. In August 2016, Dr. Nho joined the FDA's National Center for Toxicological Research (NCTR) as an Oak Ridge Institute for Science and Education fellow, and became a staff fellow in the Division of Microbiology in August 2020. He received the FDA Leveraging Collaboration Award in 2019.
Research Interests
Dr. Nho's research revolves around employing molecular and omics methods to investigate the intricate interactions between viruses and host cells. These interactions not only enable viruses to exploit cells for their own propagation, but also provide avenues for host cells to combat viral infections.
Dr. Nho has conducted research on mass spectrometry (MS)-based peptidome analysis in the serum of COVID-19 patients. This innovative approach has yielded valuable insights into virus entry and host-response interactions, with applications extending to clinical settings. By elucidating interactions between compounds and proteins, Dr. Nho's research contributes to the development of novel strategies for drug discovery. Additionally, his investigations into COVID-19-associated biomarkers using quantitative assays hold promise for enhancing patient triage, evaluating disease progression, and guiding the development of peptide antiviral therapies.
Dr. Nho has established human bronchial/tracheal and small airway epithelial cell air-liquid interface (ALI) cultures for comprehensive investigations. These cultures closely mimic the in vivo environments of human airway epithelial cells, thereby serving as invaluable tools for disease modeling and advancing ex vivo models. Through his studies on differences in cell viability, viral replication efficiency, and immune activity upon inoculation with human coronavirus (HCoV)-NL63, Dr. Nho's research sheds light on viral pathogenesis and host-immune responses, laying the groundwork for the development of therapeutic interventions against coronaviruses.
Furthermore, Dr. Nho and his colleagues have played a pivotal role in cloning and expressing spike proteins of various coronaviruses, including SARS-CoV-2, HCoV-NL-63, and HCoV-HUK1, using the insect cell-baculovirus expression vector system (IC-BEVS). This system, renowned for its efficacy in producing biological products, has facilitated the development of solutions to address emerging human diseases. The generation of polyclonal antibodies against spike proteins and their cross-reactivity analysis offer valuable insights into the mechanisms underlying coronavirus cell entry, contributing to our understanding of viral pathogenesis and aiding in the development of therapeutic strategies.
Professional Societies/National and International Groups
American Society for Microbiology
Member
2017 – Present
American Society for Virology
Member
2021 – Present
Selected Publications
Microbiological Survey of 47 Permanent Makeup Inks Available in the United States.
Yoon S., Kondakala S., Nho S.W., Moon M.S., Huang M.C.J., Periz G., Kweon O., and Kim S.
Microorganisms. 2022, 10(4):820. doi: 10.3390/microorganisms10040820.
Serotype-Identifying Ions in Listeria monocytogenes Using Matrix-Associated Laser Desorption Ionization-Time of Flight Mass Spectrometry.
Abdelhamed H., Nho S.W., Kim S.W., Reddy J.S., Park S.B., Jung T.S., and Lawrence M.L.
Heliyon. 2022, 8(12):e11769. doi: 10.1016/j.heliyon.2022.e11769.
Phylogenetically Diverse Bacteria Isolated From Tattoo Inks, an Azo Dye-Rich Environment, Decolorize a Wide Range of Azo Dyes.
Nho S.W., Cui X., Kweon O., Jin J., Chen H., Moon M.S., Kim S.J., and Cerniglia C.E.
Ann Microbiol. 2021, 71(1):10.1186/s13213-021-01648-2. doi: 10.1186/s13213-021-01648-2.
Pragmatic Strategy for Fecal Specimen Storage and the Corresponding Test Methods for Clostridioides difficile Diagnosis.
Nho S.W., Kim M., Kim S.J., Foley S.L., Nayak R., Kweon O., and Cerniglia C.E.
Pathogens. 2021, 10(8):1049. doi: 10.3390/pathogens10081049.
Microbial Contamination of Tattoo and Permanent Makeup Inks Marketed in the US: A Follow-Up Study.
Nho S.W., Kim M., Kweon O., Kim S.J., Moon M.S., Periz G., Huang M.J., Dewan K., Sadrieh N.K., and Cerniglia C.E.
Lett Appl Microbiol. 2020, 71(4):351-358. doi: 10.1111/lam.13353.
CYPminer: An Automated Cytochrome P450 Identification, Classification, and Data Analysis Tool for Genome Data Sets Across Kingdoms.
Kweon O., Kim S.J., Kim J.H., Nho S.W., Bae D., Chon J., Hart M., Baek D.H., Kim Y.C., Wang W., Kim S.K., Sutherland J.B., and Cerniglia C.E.
BMC Bioinformatics. 2020, 21(1):160. doi: 10.1186/s12859-020-3473-2.
Taxonomic and Functional Metagenomic Profile of Sediment From a Commercial Catfish Pond in Mississippi.
Nho S.W., Abdelhamed H., Paul D., Park S., Mauel M.J., Karsi A., and Lawrence M.L.
Front Microbiol. 2018, 9:2855. doi: 10.3389/fmicb.2018.02855.
Microbiological Survey of Commercial Tattoo and Permanent Makeup Inks Available in the United States.
Nho S.W., Kim S.J., Kweon O., Howard P.C., Moon M.S., Sadrieh N.K., and Cerniglia C.E.
J Appl Microbiol. 2018, 124(5):1294-1302. doi: 10.1111/jam.13713.
Improving Safety of a Live Attenuated Edwardsiella ictaluri Vaccine Against Enteric Septicemia of Catfish and Evaluation of Efficacy.
Nho S.W., Abdelhamed H., Karsi A., and Lawrence M.L.
Vet Microbiol. 2017, 210:83-90. doi: 10.1016/j.vetmic.2017.09.004.
Evaluation of Three Recombinant Outer Membrane Proteins, OmpA1, Tdr, and TbpA, as Potential Vaccine Antigens Against Virulent Aeromonas hydrophila Infection in Channel Catfish (Ictalurus punctatus).
Abdelhamed H., Ibrahim I., Nho S.W., Banes M.M., Wills R.W., Karsi A., and Lawrence M.L.
Fish Shellfish Immunol. 2017, 66:480-486. doi: 10.1016/j.fsi.2017.05.043.
Comparative Genomics and Transcriptional Analysis of Flavobacterium columnare Strain ATCC 49512.
Tekedar H.C., Karsi A., Reddy J.S., Nho S.W., Kalindamar S., and Lawrence M.L.
Front Microbiol. 2017, 8:588. doi: 10.3389/fmicb.2017.00588.
Characterization of a Specific Monoclonal Antibody Against Immunoglobulin Light Kappa/L1 Chain in Olive Flounder (Paralichthys olivaceus).
Kim Y.K., Lee J.S., Jung J.W., Hikima J.I., Ohtani M., Jang H.B., Nho S.W., Cha I.S., Park S.B., Lee J.H., Aoki T., and Jung T.S.
Fish Shellfish Immunol. 2017, 60:88-96. doi: 10.1016/j.fsi.2016.11.023.
Development of an Immunochromatography Assay Kit for Rapid Detection of Ranavirus.
Kim Y.R., Park S.B., Fagutao F.F., Nho S.W., Jang H.B., Cha I.S., Thompson K.D., Adams A., Bayley A., and Jung T.S.
J Virol Methods. 2015, 223:33-9. doi: 10.1016/j.jviromet.2015.07.009.
Identification of High-Risk Listeria monocytogenes Serotypes in Lineage I (Serotype 1/2a, 1/2c, 3a and 3c) Using Multiplex PCR.
Nho S.W., Abdelhamed H., Reddy S., Karsi A., and Lawrence M.L.
J Appl Microbiol. 2015, 119(3):845-52. doi: 10.1111/jam.12876.
Comparative Genomic Characterization of Three Streptococcus parauberis Strains in Fish Pathogen, as Assessed by Wide-Genome Analyses.
Nho S.W., Hikima J., Park S.B., Jang H.B., Cha I.S., Yasuike M., Nakamura Y., Fujiwara A., Sano M., Kanai K., Kondo H., Hirono I., Takeyama H., Aoki T., and Jung T.S.
PLoS One. 2013, 8(11):e80395. doi: 10.1371/journal.pone.0080395.
Heat Shock Protein Profiles on the Protein and Gene Expression Levels in Olive Flounder Kidney Infected with Streptococcus parauberis.
Cha I.S., Kwon J., Park S.B., Jang H.B., Nho S.W., Kim Y.K., Hikima J., Aoki T., and Jung T.S.
Fish Shellfish Immunol. 2013, 34(6):1455-62. doi: 10.1016/j.fsi.2013.03.355.
RNA-Seq-Based Metatranscriptomic and Microscopic Investigation Reveals Novel Metalloproteases of Neobodo sp. as Potential Virulence Factors for Soft Tunic Syndrome in Halocynthia roretzi.
Jang H.B., Kim Y.K., Del Castillo C.S., Nho S.W., Cha I.S., Park S.B., Ha M.A., Hikima J., Hong S.J., Aoki T., and Jung T.S.
PLoS One. 2012, 7(12):e52379. doi: 10.1371/journal.pone.0052379.
Complete Genome Sequence and Immunoproteomic Analyses of the Bacterial Fish Pathogen Streptococcus parauberis.
Nho S.W., Hikima J., Cha I.S., Park S.B., Jang H.B., del Castillo C.S., Kondo H., Hirono I., Aoki T., and Jung T.S.
J Bacteriol. 2011, 193(13):3356-66. doi: 10.1128/JB.00182-11.
Comparative Sequence Analysis of a Multidrug-Resistant Plasmid from Aeromonas hydrophila.
Del Castillo C.S., Hikima J., Jang H.B., Nho S.W., Jung T.S., Wongtavatchai J., Kondo H., Hirono I., Takeyama H., and Aoki T.
Antimicrob Agents Chemother. 2013, 57(1):120-9. doi: 10.1128/AAC.01239-12.
Enhanced Reliability of Avian Influenza Virus (AIV) and Newcastle Disease Virus (NDV) Identification Using Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry (MALDI-MS).
Jang H.B., Sung H.W., Nho S.W., Park S.B., Cha I.S., Aoki T., and Jung T.S.
Anal Chem. 2011, 83(5):1717-25. doi: 10.1021/ac102846q.
Innate Immune Response in the Hemolymph of an Ascidian, Halocynthia roretzi, Showing Soft Tunic Syndrome, Using Label-Free Quantitative Proteomics.
Cha I.S., Castillo C.S., Nho S.W., Hikima J., Aoki T., and Jung T.S.
Dev Comp Immunol. 2011, 35(8):809-16. doi: 10.1016/j.dci.2011.01.011.
Lab Members
Contact information for all lab members:
(870) 543-7391
NCTRResearch@fda.hhs.gov
Marli Azevedo, Ph.D.
Research biologist
Lisa Mullis
Biologist
- Contact Information
- Seongwon Nho
- (870)543-7121
- Expertise
-
ExpertiseApproachDomainTechnology & DisciplineToxicology