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  8. Lei Guo
  1. Science & Research (NCTR)

Lei Guo Ph.D.

Biologist, Senior Biomedical Research and Biomedical Product Assessment Service Expert — Division of Biochemical Toxicology

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Lei Guo, Ph.D.
(870) 543-7121
NCTRResearch@fda.hhs.gov

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About  |  Publications  |  Lab Members

Background

Lei Guo received a Ph.D. in molecular biology from the University of Tokyo (Japan) and worked as a postdoctoral fellow and then as a research assistant professor at the University of Alberta (Canada) for four years. Dr. Guo joined FDA’s National Center for Toxicological Research (NCTR) in 2003. She has published more than 100 articles in peer-reviewed journals and 16 book chapters. Her work has been cited more than 10,000 times.

 

Research Interests

Dr. Guo's research focuses on mechanistic studies of and herbal dietary supplement- and drug-associated toxicities. She uses multiple in vitro models and approaches, including, but not restricted to, molecular and biochemical methods, genomic analyses, and in silico molecular docking for evaluation and prediction of toxicity and elucidation of toxic mechanisms. Her research results demonstrate that this combined approach is useful for hazard identification and that in-depth molecular mechanistic studies can provide toxicological information for FDA-relevant products.

 

Professional Societies/National and International Groups

International Agency for Research on Cancer/World Health Organization Monographs on the Evaluation of Carcinogenic Risks to Humans
Committee member
2020

National Institute of Environmental Health Sciences P42 Superfund
Grant Review Member
2019

Society of Toxicology (SOT)
Member
2004 – Present
Chair, Board of Publication
2022



Selected Publications

Evaluation of Weak Genotoxicity of Hydroxychloroquine in Human TK6 Cells.
Li X., Le Y., Li Y., Chen S., Guo L., Fu X., Manjanatha M.G., and Mei N.
Toxicol Lett. 2024, 393:84-95.

The Involvement of Hepatic Cytochrome P450s in the Cytotoxicity of Lapatinib.
Chen S., Li X., Li Y., He X., Bryant M., Qin X., Li F., Seo J.E., Guo X., Mei N., and Guo L.
Toxicol Sci. 2023, 197(1):69-78.  

Identifying the Reactive Metabolites of Tyrosine Kinase Inhibitor Pexidartinib In Vitro Using LC-MS-Based Metabolomic Approaches.
Qin X., Wang Y., MacKenzie K.R., Hakenjos J.M., Chen S., Khalil S.M., Jung S.Y., Young D.W., Guo L., and Li F.
Chem Res Toxicol. 2023, 36(8):1427-1438.

Cannabidiol-Induced Transcriptomic Changes and Cellular Senescence in Human Sertoli Cells.
Li Y., Li X., Cournoyer P., Choudhuri S., Yu X., Guo L., and Chen S.
Toxicol Sci. 2023, 191(2):227-238.

Development of Benchmark Datasets for Text Mining and Sentiment Analysis to Accelerate Regulatory Literature Review.
Wu L., Chen S., Guo L., Shpyleva S., Harris K., Fahmi T., Flanigan T., Tong W., Xu J., and Ren Z.
Regul Toxicol Pharmacol. 2023, 137:105287.

Study of the Roles of Cytochrome P450 (CYPs) in the Metabolism and Cytotoxicity of Perhexiline.
Ren Z., Chen S., Qin X., Li F., and Guo L.
Arch Toxicol. 2022, 96(12):3219-3231.

The Expression of Phase II Drug-Metabolizing Enzymes in Human B-Lymphoblastoid TK6 Cells.
Li X., Li Y., Ning K.G., Chen S., Guo L., Bonzo J.A., and Mei N.
J Environ Sci Health C Toxicol Carcinog. 2022, 40(1):106-118.

In Vitro Effects of Cannabidiol and Its Main Metabolites in Mouse and Human Sertoli Cells.
Li Y., Wu Q., Li X., Von Tungeln L.S., Beland F.A., Petibone D., Guo L., Cournoyer P., Choudhuri S., and Chen S.
Food Chem Toxicol. 2022, 159:112722.

Roles of CYP3A4, CYP3A5 and CYP2C8 Drug-Metabolizing Enzymes in Cellular Cytostatic Resistance.
Hofman J., Vagiannis D., Chen S., and Guo L.
Chem Biol Interact. 2021, 340:109448.

The Genotoxicity Potential of Luteolin is Enhanced by CYP1A1 and CYP1A2 in Human Lymphoblastoid TK6 Cells.
Li X., He X., Chen S., Le Y., Bryant M.S., Guo L., Witt K.L., and Mei N.
Toxicol Lett. 2021, 344:58-68.

Characterization of Cytochrome P450s (CYP)-Overexpressing HepG2 Cells for Assessing Drug and Chemical-Induced Liver Toxicity.
Chen S., Wu Q., Li X., Li D., Mei N., Ning B., Puig M., Ren Z., Tolleson WH., and Guo L.
J Environ Sci Health C Toxicol Carcinog. 2021, 39(1):68-86.

A Mechanism of Perhexiline's Cytotoxicity in Hepatic Cells Involves Endoplasmic Reticulum Stress and p38 Signaling Pathway.
Ren Z., Chen S., Pak S., and Guo L.
Chem Biol Interact. 2021, 334:109353.

Mitochondrial Dysfunction and Apoptosis Underlie the Hepatotoxicity of Perhexiline.
Ren Z., Chen S., Seo J.E., Guo X., Li D., Ning B., and Guo L.
Toxicol In Vitro. 2020, 69:104987.

Evaluation of Pyrrolizidine Alkaloid-Induced Genotoxicity Using Metabolically Competent TK6 Cell Lines.
Li X., He X., Chen S., Le Y., Guo X., Bryant M.S., Guo L., Manjanatha M.G., Zhou T., Witt K.L., and Mei N.
Food Chem Toxicol. 2020, 145:111662.

The Role of Hepatic Cytochrome P450s in the Cytotoxicity of Sertraline.
Chen S., Wu Q., Li X., Li D., Fan M., Ren Z., Bryant M., Mei N., Ning B., and Guo L. 
Arch Toxicol. 2020, 94(7):2401-2411.

Ensartinib (X-396) Effectively Modulates Pharmacokinetic Resistance Mediated by ABCB1 and ABCG2 Drug Efflux Transporters and CYP3A4 Biotransformation Enzyme.
Vagiannis D., Novotna E., Skarka A., Kammerer S., Küpper J.H., Chen S., Guo L., Staud F., and Hofman J.
Cancers (Basel). 2020, 12(4):813.

Long Noncoding RNA LINC00844-Mediated Molecular Network Regulates Expression of Drug Metabolizing Enzymes and Nuclear Receptors in Human Liver Cells.
Li D., Wu L., Knox B., Chen S., Tolleson W.H., Liu F., Yu D., Guo L., Tong W., and Ning B.
Arch Toxicol. 2020 May;94(5):1637-1653.

Development and Application of TK6-derived Cells Expressing Human Cytochrome P450s for Genotoxicity Testing.
Li X., Chen S., Guo X., Wu Q., Seo J.E., Guo L., Manjanatha M.G., Zhou T., Witt K.L., and Mei N.
Toxicol Sci. 2020, 175(2):251-265.

Methods for Establishing and Using a Stable Cell Line Expressing Both Gaussia Luciferase and Firefly Luciferase to Screen for Endoplasmic Reticulum Stress.
Ren Z., Chen S., and Guo L.
Methods Mol Biol. 2020, 2102:531-555.

Using a Lentivirus-Based Inducible RNAi Vector to Silence a Gene.
Chen S., Li D., Ren Z., Yu D., Ning B., Mei N., and Guo L.
Methods Mol Biol. 2020, 2102:195-210.

 

Lab Members

Contact information for all lab members:
(870) 543-7121
NCTRResearch@fda.hhs.gov

Si Chen, Ph.D.
Research Biologist

Yuxi Li, Ph.D.
FDA Staff Fellow

Contact Information
Lei Guo
(870) 543-7121
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