Serum-Free Medium for the Isolation and Expansion of Canine Adipose-Derived Mesenchymal Stem/Stromal Cells
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Mesenchymal Stem/Stromal Cells (MSCs) are a potentially effective therapy for treating a variety of conditions in veterinary medicine. MSCs are isolated and expanded in vitro in a medium that is supplemented with fetal bovine serum (FBS). However, the use of FBS as a medium component during manufacturing may pose several problems. FBS can be a source of unwanted pathogens, the serum can introduce carry-over components that may elicit an immune response, and lot-to-lot variations in FBS quality may introduce inconsistencies between batches of MSCs.
This invention is a novel FBS-free medium that supports both the isolation and expansion of MSCs from canine adipose tissue while removing the unwanted pathogens, immune response and lot-to-lot variations.
|Potential Commercial Applications||Competitive Advantages|
Development Stage: Research Material
Inventors: Lax Devireddy, Michael Myers, Rudell Screven
- Devireddy, LR., et. al. A serum-free medium formulation efficiently supports isolation and propagation of canine adipose-derived mesenchymal stem/stromal cells. PLoS One. 2019 Feb 27;14(2) PMID: 30811421
Product Area: research material, supplement, media, serum
FDA Reference No: E-2017-010
Ken Millburne, J.D.
FDA Technology Transfer Program