Direct Impact Spark Ionization (DISI) Mass Spectrometry (MS) for Identification of Microbes
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Generating reproducible mass spectra from bacterial samples at atmospheric pressure is challenging. FDA/NCTR inventors designed a rapid mass spectrometry device using direct impact spark ionization source for identification of microbial analytes using spectral pattern recognition.
The device’s design includes a rapid mass spectrometer suitable for analyzing microbiological samples that was previously used to analyze low volatility organic compounds. The device uses a solid needle for electrode discharge. It includes a gear plate that introduces stainless steel pins carrying bacterial samples. The pins also act as counter electrodes that are targeted by controlled arcs. The small custom-made glass cylinder is meant to shut out oxygen and prevent the introduction of ambient moisture into the analyte is unique from other DISI devices. Studies revealed enormous peak intensity and spectral information with normal ionization mode on the same instrument. This device can be used for pathogen determination in clinical settings, QA/QC (drugs, food or cosmetic ingredients), along with continuous monitoring of (airborne) Biological Warfare Agents.
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Inventors: Jon Wilkes, Pierre Alusta, Cameron Dorey, Ryan Parker, Dan Buzatu
Alusta P, Buzatu D, Williams A, et al. Instrumental improvements and sample preparations that enable reproducible, reliable acquisition of mass spectra from whole bacterial cells. Rapid Commun Mass Spectrom. 2015;29(21):1961‐1968. PMID: 26445594
United States Patent No. 8,704,169 issued 04.22.2014
Product Area: Pathogen detection, biodefense, airborne detection CBRN, Quality control/assurance
FDA Reference No: E-2011-021
Ken Millburne, J.D.
FDA Technology Transfer Program