An Attenuated Live Mumps Virus Vaccine Candidate Expressing F and HN Protein Genes from Genotype G
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Despite near elimination of mumps in the US and other countries that immunize against mumps, there has been a resurgence of the disease, even in highly vaccinated populations. This has resulted in calls for development of new, more efficacious mumps vaccines. However, attenuation of mumps viruses has proven difficult, resulting in the use of some vaccine strains later identified as causing aseptic meningitis in recipients. This has led to vaccine withdrawal, public resistance to vaccination, and even cessation of immunization programs in some countries. There is a need for the development of newer, more efficacious mumps vaccines to address the growing cases of a once dormant disease.
This invention is a mumps vaccine candidate engineered for easy adaptability to match changes in the circulating strains, a robust immune response for lasting immunity, and a key mutation to reduce virulence and maintain attenuation. This mumps vaccine candidate is a Jeryl Lynn-based, recombinant virus construct that expresses the genotype G fusion (F) and hemagglutinin-neuraminidase (HN) proteins of circulating mumps strains. It includes a mutation in the viral protein V gene to prevent the expression of a key virulence factor and two restriction enzyme recognition sites to permit efficient exchange of the F and HN protein genes to accommodate the changes in the genotype of the circulating mumps virus. Using in vivo models, it was determined that (1) the HN and F proteins are the major drivers of the anti-mumps virus immune response and (2) matching the vaccine F and HN proteins to those of currently circulating strains would likely improve the duration of immunity. The candidate vaccine demonstrated proper attenuation to avoid aseptic meningitis in the animal model studies. The recombinant mumps virus can potentially be used as a vaccine to inhibit mumps virus infection and the development of the mumps disease.
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Inventors: Steven Rubin, Trent J. Bosma, Christian J. Sauder, Tahir H. Malik
- Lemon, K., et. al. 2007. 'The F gene of rodent brain-adapted mumps virus is a major determinant of neurovirulence', J Virol, 81: 8293-302. PMID: 17475640
- Malik, T.H., et. al. 2007. 'A single nucleotide change in the mumps virus F gene affects virus fusogenicity in vitro and virulence in vivo', J Neurovirol, 13: 513-21. PMID: 18097883
- Rubin, S.A., et. al. 2012. 'Recent mumps outbreaks in vaccinated populations: no evidence of immune escape', J Virol, 86: 615-20. PMID: 22072778
- Sauder, C.J., et. al. 2011. 'Gene-specific contributions to mumps virus neurovirulence and neuroattenuation', J Virol, 85: 7059-69. PMID: 21543475
Intellectual Property: PCT/US2019/065926 filed 12/12/2019
Product Area: mumps, combination vaccine, Jeryl Lynn, infectious, genotype, immunity, fusion, strain, hemagglutinin, neuraminidase, protein, mutation, viral, protein, disease, attenuation
FDA Reference No: E-2018-022
Ken Millburne, J.D.
FDA Technology Transfer Program