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  1. Laboratory Methods (Food)

BAM Media M190: Vibrio vulnificus Agar (VVA)

May 2004

Vibrio vulnificus Agar (VVA)

Solution 1

Peptone 20 g
NaCl 30 g
100X Dye stock solution* 10 ml
Agar 25 g
Distilled water 900 ml

Adjust to pH 8.2. Boil to dissolve agar. Autoclave for 15 min at 121°C. Temper to 50°C.

*100× Dye stock solution

Bromthymol blue 0.6 g
70% Ethanol 100 ml

For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes.

Solution 2

Cellobiose 10 g
Distilled water 100 ml

Dissolve cellobiose in distilled water by heating gently. Cool and filter sterilize.

Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, light blue.

Medium may be stored 2 weeks at refrigeration temperatures.

Storage: limit: 2 wks Temperature: 4°C


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